A comparative biochemical study of the interaction of some trypanocides with rat tissue cellular systems

The effects of three trypanocides (tryparsamide, novidium and berenil) on rat liver and kidney were studied with a view to understanding the site of primary injury to the cell resulting from their administration. Four enzymes which are exclusively located in specific regions of the cell were used...

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Egile nagusia: Akanji, Musbau Adewumi
Beste egile batzuk: Edwin.O. Ngaha, J.O. Folahan
Formatua: Thesis
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Argitaratua: Obafemi Awolowo University, Ile Ife, Nigeria 2013
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author Akanji, Musbau Adewumi
author2 Edwin.O. Ngaha, J.O. Folahan
author_facet Edwin.O. Ngaha, J.O. Folahan
Akanji, Musbau Adewumi
author_sort Akanji, Musbau Adewumi
collection DSpace
description The effects of three trypanocides (tryparsamide, novidium and berenil) on rat liver and kidney were studied with a view to understanding the site of primary injury to the cell resulting from their administration. Four enzymes which are exclusively located in specific regions of the cell were used to monitor the regions affected by the drugs. The results obtained showed that administration of each of the three drugs resulted in massive increase of alkaline phosphates activity and a very marked inhibition of lactate dehydrogenase activity in both tissues. There was no significant effect on the lysosomal enzymes both in vivo and in vitro. These results indicate that tryparsamide, novidium and berenil ellicit their action on the cellular system of both liver and kidney in similar ways when administered to rats. Inhibition of lactate dehydrogenase activity which is located in the soluble fraction of cytoplasm may lead to accumulation of pyruvate in the cell. The massive increase of alkaline phosphates activity in the tissues may also lead to indiscriminate hydrolysis of phosphate esters needed for vital activities in the cell.
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language English
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publisher Obafemi Awolowo University, Ile Ife, Nigeria
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spelling oai:ir.oauife.edu.ng:123456789-32942023-05-13T11:08:29Z A comparative biochemical study of the interaction of some trypanocides with rat tissue cellular systems Akanji, Musbau Adewumi Edwin.O. Ngaha, J.O. Folahan Indiscrimainate Hydrolysis Phosphate Esters Trypanocides Lactate Dehydrogenase The effects of three trypanocides (tryparsamide, novidium and berenil) on rat liver and kidney were studied with a view to understanding the site of primary injury to the cell resulting from their administration. Four enzymes which are exclusively located in specific regions of the cell were used to monitor the regions affected by the drugs. The results obtained showed that administration of each of the three drugs resulted in massive increase of alkaline phosphates activity and a very marked inhibition of lactate dehydrogenase activity in both tissues. There was no significant effect on the lysosomal enzymes both in vivo and in vitro. These results indicate that tryparsamide, novidium and berenil ellicit their action on the cellular system of both liver and kidney in similar ways when administered to rats. Inhibition of lactate dehydrogenase activity which is located in the soluble fraction of cytoplasm may lead to accumulation of pyruvate in the cell. The massive increase of alkaline phosphates activity in the tissues may also lead to indiscriminate hydrolysis of phosphate esters needed for vital activities in the cell. 2013-08-19T14:51:43Z 2018-10-29T10:55:57Z 2013-08-19T14:51:43Z 2018-10-29T10:55:57Z 1986 Thesis Akanji, Musbau Adewumi (1986) A comparative biochemical study of the interaction of some trypanocides with rat tissue cellular systems. Ph.D. Biochemistry, Ile-Ife: OAU http://localhost:8080/xmlui/handle/123456789/3294 en PDF application/pdf Nigeria Obafemi Awolowo University, Ile Ife, Nigeria
spellingShingle Indiscrimainate Hydrolysis
Phosphate Esters
Trypanocides
Lactate Dehydrogenase
Akanji, Musbau Adewumi
A comparative biochemical study of the interaction of some trypanocides with rat tissue cellular systems
title A comparative biochemical study of the interaction of some trypanocides with rat tissue cellular systems
title_full A comparative biochemical study of the interaction of some trypanocides with rat tissue cellular systems
title_fullStr A comparative biochemical study of the interaction of some trypanocides with rat tissue cellular systems
title_full_unstemmed A comparative biochemical study of the interaction of some trypanocides with rat tissue cellular systems
title_short A comparative biochemical study of the interaction of some trypanocides with rat tissue cellular systems
title_sort comparative biochemical study of the interaction of some trypanocides with rat tissue cellular systems
topic Indiscrimainate Hydrolysis
Phosphate Esters
Trypanocides
Lactate Dehydrogenase
url http://localhost:8080/xmlui/handle/123456789/3294
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