Induction of Interferon by Poly A: Poly U in Primary Tissue Cultures.
Primary tissue culture was grown using bat (Eidolon helvum) breast muscle. These tissue cultures were treated with polyadenylic acid: polyuridylic acid) polyA: polyU). After 24 hours the culture brew was harvested and from this, interferon was extracted and purified to a specific activity of 1.5 x 1...
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| 格式: | Thesis |
| 语言: | 英语 |
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Obafemi Awolowo University
2014
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| 在线阅读: | http://localhost:8080/xmlui/handle/123456789/3296 |
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| 总结: | Primary tissue culture was grown using bat (Eidolon helvum) breast muscle. These tissue cultures were treated with polyadenylic acid: polyuridylic acid) polyA: polyU). After 24 hours the culture brew was harvested and from this, interferon was extracted and purified to a specific activity of 1.5 x 105 IFN Units/mg protein. The interferon was shown to have a molecular weight of 24,000 and fully protected bats' cells from the action of Wesselsbron Virus (H10964). |
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