Post-infectional Production of Phytoalexin in White Yam (Discorea Rotundata Linn.) Innoculated with Botryodiplodia Theobromae Pat
Four phytoalexins were induced D. rotundata in post-infectionally with B. theobromae. Three of the Phytoalexins were isolated and identified with the aid of ultra-violet mass and nuclear magnetic resonance spectral data as 3,2,5 trihydroxydiphenyl 1 , 2 ethane; 3, 5 dihydroxydiphenyl 1,2 ethane; 1,5...
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Obafemi Awolowo University
2014
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| _version_ | 1810764577487454208 |
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| author | Fagbohun, Dayo Emman |
| author2 | Ogundana, S. K. |
| author_facet | Ogundana, S. K. Fagbohun, Dayo Emman |
| author_sort | Fagbohun, Dayo Emman |
| collection | DSpace |
| description | Four phytoalexins were induced D. rotundata in post-infectionally with B. theobromae. Three of the Phytoalexins were isolated and identified with the aid of ultra-violet mass and nuclear magnetic resonance spectral data as 3,2,5 trihydroxydiphenyl 1 , 2 ethane; 3, 5 dihydroxydiphenyl 1,2 ethane; 1,5 dihydroxy-3-methoxydiphenyl 1,2 ethane and an uncharacterised.
Maximum production of the phytoalexins was achieved at 30 hours of incubation. Chloroform: methanol, (96:4 v/v) gave the best separation of the phytoalexins from yam crude extract. Antifungal activities of the phytoalexins against spore germination varied according to fungal species but the pores were more sensitive to 3, 2, 5 trihydroxydiphenyl 1,2 ethane than 3, 5 dihydroxydiphenyl 1,2 ethane and the spores of B. theobromae were least affected.
The ED50 values required to prevent spore germination of the pathogen varied from 45.7 µg/ml 58.9 µg/ml for 3,2, 5 trihydroxydiphenyl 1,2 ethane and 45.6 µg/ml to 81.3 µg/ml ;3,5 dihydroxydiphenyl 1,2 ethane. The ED50 value of the two phytoalexins to prevent germ tube elongation of the yam rot pathogens was highest for B. theobromae (61.07 µg/ml) and least for A.niger (46.77 µg/ml). The compound 3,2, 5 trihydroxydiphenyl 1 ,2 ethane was found to be active against the four bacterial species tested against it even at the low concentration of 10 µg/ml. |
| format | Thesis |
| id | oai:ir.oauife.edu.ng:123456789-3516 |
| institution | My University |
| language | English |
| publishDate | 2014 |
| publisher | Obafemi Awolowo University |
| record_format | dspace |
| spelling | oai:ir.oauife.edu.ng:123456789-35162023-05-13T11:11:29Z Post-infectional Production of Phytoalexin in White Yam (Discorea Rotundata Linn.) Innoculated with Botryodiplodia Theobromae Pat Fagbohun, Dayo Emman Ogundana, S. K. Infection phytoalexins ultra-violet mass nuclear magnetic resonance spectral data incubation chloroform methanol yam crude extract Four phytoalexins were induced D. rotundata in post-infectionally with B. theobromae. Three of the Phytoalexins were isolated and identified with the aid of ultra-violet mass and nuclear magnetic resonance spectral data as 3,2,5 trihydroxydiphenyl 1 , 2 ethane; 3, 5 dihydroxydiphenyl 1,2 ethane; 1,5 dihydroxy-3-methoxydiphenyl 1,2 ethane and an uncharacterised. Maximum production of the phytoalexins was achieved at 30 hours of incubation. Chloroform: methanol, (96:4 v/v) gave the best separation of the phytoalexins from yam crude extract. Antifungal activities of the phytoalexins against spore germination varied according to fungal species but the pores were more sensitive to 3, 2, 5 trihydroxydiphenyl 1,2 ethane than 3, 5 dihydroxydiphenyl 1,2 ethane and the spores of B. theobromae were least affected. The ED50 values required to prevent spore germination of the pathogen varied from 45.7 µg/ml 58.9 µg/ml for 3,2, 5 trihydroxydiphenyl 1,2 ethane and 45.6 µg/ml to 81.3 µg/ml ;3,5 dihydroxydiphenyl 1,2 ethane. The ED50 value of the two phytoalexins to prevent germ tube elongation of the yam rot pathogens was highest for B. theobromae (61.07 µg/ml) and least for A.niger (46.77 µg/ml). The compound 3,2, 5 trihydroxydiphenyl 1 ,2 ethane was found to be active against the four bacterial species tested against it even at the low concentration of 10 µg/ml. 2014-06-25T09:45:14Z 2018-10-29T11:18:09Z 2014-06-25T09:45:14Z 2018-10-29T11:18:09Z 1986 Thesis APA http://localhost:8080/xmlui/handle/123456789/3516 en pdf application/pdf Obafemi Awolowo University |
| spellingShingle | Infection phytoalexins ultra-violet mass nuclear magnetic resonance spectral data incubation chloroform methanol yam crude extract Fagbohun, Dayo Emman Post-infectional Production of Phytoalexin in White Yam (Discorea Rotundata Linn.) Innoculated with Botryodiplodia Theobromae Pat |
| title | Post-infectional Production of Phytoalexin in White Yam (Discorea Rotundata Linn.) Innoculated with Botryodiplodia Theobromae Pat |
| title_full | Post-infectional Production of Phytoalexin in White Yam (Discorea Rotundata Linn.) Innoculated with Botryodiplodia Theobromae Pat |
| title_fullStr | Post-infectional Production of Phytoalexin in White Yam (Discorea Rotundata Linn.) Innoculated with Botryodiplodia Theobromae Pat |
| title_full_unstemmed | Post-infectional Production of Phytoalexin in White Yam (Discorea Rotundata Linn.) Innoculated with Botryodiplodia Theobromae Pat |
| title_short | Post-infectional Production of Phytoalexin in White Yam (Discorea Rotundata Linn.) Innoculated with Botryodiplodia Theobromae Pat |
| title_sort | post infectional production of phytoalexin in white yam discorea rotundata linn innoculated with botryodiplodia theobromae pat |
| topic | Infection phytoalexins ultra-violet mass nuclear magnetic resonance spectral data incubation chloroform methanol yam crude extract |
| url | http://localhost:8080/xmlui/handle/123456789/3516 |
| work_keys_str_mv | AT fagbohundayoemman postinfectionalproductionofphytoalexininwhiteyamdiscorearotundatalinninnoculatedwithbotryodiplodiatheobromaepat |