Synthesis charactrization, in vitro antimicrobial and antioxidant activites of chalcone derivatives of 4-hydroxyacetophenone

xix,120

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Yazar: Kelani, Monsuru Temitope
Materyal Türü: Tez
Dil:İngilizce
Baskı/Yayın Bilgisi: Obafemi Awolowo University 2019
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Online Erişim:https://ir.oauife.edu.ng/handle/123456789/4126
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author Kelani, Monsuru Temitope
author_facet Kelani, Monsuru Temitope
author_sort Kelani, Monsuru Temitope
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description xix,120
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publishDate 2019
publisher Obafemi Awolowo University
record_format dspace
spelling oai:ir.oauife.edu.ng:123456789-41262023-05-13T11:29:58Z Synthesis charactrization, in vitro antimicrobial and antioxidant activites of chalcone derivatives of 4-hydroxyacetophenone Kelani, Monsuru Temitope Synthesis in vitro Antimicrobial Characterization in Vitro antimicrobial Antioxidant Activities of Chalcone Derivatives 4-Hydroxyacetophenone Synthesis Antioxidant Chalcones Antimicrobial xix,120 This study synthesizedsome phenolic chalcone-ninhydrin adducts via microwave-irradiation of phenolic chalcones with ninhydrin. This was with a view to improving the chemotherapeutic property of chalcones. The chalcones were prepared by reacting 4-hydroxyacetophenone with an equivalent mole of sevensubstituted benzaldehydesto afford the corresponding chalcones (1a, 2a, 3a, 4a, 5a, 6a and 7a). The resulting phenolic-chalcones were reacted with ninhydrin by pulse microwave-irradiationof the reactants in glacial acetic acid for 8 min to give the following compounds 1b, 2b, 3b, and 4b, except the reaction of 7a with ninhydrin which was achieved by refluxing in glacial acetic acid for 6 hours to yieldninhydrin adduct of the chacone7b.The compounds were screened for antioxidant activities which included2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) and (ferric reducing antioxidant power)FRAP assays. They were also screened in-vitro against nine strains of Gram-positive and five Gram-negative bacteria, to determine their zones of inhibition, minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC). The structures of some of the synthesized compounds were investigated and confirmed using nuclear magnetic resonance (NMR) experiments (1H, 13C, and distortionless enhancement by polarization transfer (DEPT). Some of the compounds were also subjected to infra-red spectroscopic analysis and mass spectrometry to corroborate the information obtained from the NMR experiments. The spectroscopic analysis showed that the expected structures of the chalcones were obtained and that the ninhydrin adducts were either di- or tri-substituted adducts.Compound 7a demonstrated the best DPPH radical scavenging ability with an IC50 value of 0.0240 mg/ml which was lower than the standard (ascorbic acid, 0.0250 mg/ml).In the FRAP assay, however, compound 4b demonstrated the best activity with an ascorbic acid equivalent of (AAE) of 0.470 mg. Therefore,compounds 7a and 7b exhibited a comparable radical scavenging ability to the ascorbic acid used as standard drug than the other compounds against the DPPH, while compound 4b exhibited an excellent ferric reducing antioxidant ability using the FRAP assays. All the bacterial strains were sensitive to compound 5a except Pseudomonasaeruginosa,which was also not susceptible to the standard drugs, streptomycin and ampicillin. Compound 5a exhibited the best activity against the Gram-negative bacteria and also had the lowest MIC values than ampicillin. The study concluded that compounds 4a, 7a and 7b could serve as good antioxidant agents and compound 5aexhibited an excellent antimicrobial property. 2019-03-27T10:07:43Z 2019-03-27T10:07:43Z 2014 Thesis Kelani, M.T. (2014). Synthesis charactrization, in vitro antimicrobial and antioxidant activites of chalcone derivatives of 4-hydroxyacetophenone https://ir.oauife.edu.ng/handle/123456789/4126 en application/pdf Obafemi Awolowo University
spellingShingle Synthesis in vitro Antimicrobial
Characterization in Vitro antimicrobial
Antioxidant Activities of Chalcone Derivatives
4-Hydroxyacetophenone
Synthesis
Antioxidant
Chalcones
Antimicrobial
Kelani, Monsuru Temitope
Synthesis charactrization, in vitro antimicrobial and antioxidant activites of chalcone derivatives of 4-hydroxyacetophenone
title Synthesis charactrization, in vitro antimicrobial and antioxidant activites of chalcone derivatives of 4-hydroxyacetophenone
title_full Synthesis charactrization, in vitro antimicrobial and antioxidant activites of chalcone derivatives of 4-hydroxyacetophenone
title_fullStr Synthesis charactrization, in vitro antimicrobial and antioxidant activites of chalcone derivatives of 4-hydroxyacetophenone
title_full_unstemmed Synthesis charactrization, in vitro antimicrobial and antioxidant activites of chalcone derivatives of 4-hydroxyacetophenone
title_short Synthesis charactrization, in vitro antimicrobial and antioxidant activites of chalcone derivatives of 4-hydroxyacetophenone
title_sort synthesis charactrization in vitro antimicrobial and antioxidant activites of chalcone derivatives of 4 hydroxyacetophenone
topic Synthesis in vitro Antimicrobial
Characterization in Vitro antimicrobial
Antioxidant Activities of Chalcone Derivatives
4-Hydroxyacetophenone
Synthesis
Antioxidant
Chalcones
Antimicrobial
url https://ir.oauife.edu.ng/handle/123456789/4126
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