Regeneration of Adiantum Capillus-Veneris L.: an homosporous fern species.

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1. autor: Olatunji, Tosin Yetunde
Format: Praca dyplomowa
Język:angielski
Wydane: Obafemi Awolowo University 2019
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Dostęp online:https://ir.oauife.edu.ng/handle/123456789/4287
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author Olatunji, Tosin Yetunde
author_facet Olatunji, Tosin Yetunde
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spelling oai:ir.oauife.edu.ng:123456789-42872023-05-13T11:28:59Z Regeneration of Adiantum Capillus-Veneris L.: an homosporous fern species. Olatunji, Tosin Yetunde Regeneration of Adiantum Capillus-Veneris Homosporous Fern Species Spore and Tissues Vegetative Propagation Parker's and Thompson's Nutrient Fern Medicinal fern species Pteridophyte Pteridophytes Adiantum Capillus-Veneris xi,59 Spore and tissue culturestudies ofAdiantum capillus-veneris, an ornamental and medicinal fern species were carried outto raise new fernlets, study the growth rate and develop a protocol for rapid vegetative propagation of the fern in-vitro. Sterilized spores were sown on semi-solid agar containing Parker’s and Thompson’s nutrient. Agar and soil cultures were established to study spore germination and gametophyte development. The germination rate was scored at an interval of four days for thirty days.Sporophyte and gametophyte explants were sterilized and cultured onMurashige and Skoog (MS) medium supplemented with different auxins (IBA and NAA) and cytokinins (BA and Kinetin). Callus regeneration was obtained from leaflet explants cultured on MS medium supplemented with 0.50 mg/L NAA. Root regeneration was obtained from rhizome explants cultured on MS medium supplemented with 0.5 mg/L IBA and MS medium supplemented with 0.5 mg/L NAA. Shoot regeneration was obtained from stipe explant cultured on MS medium supplemented with 0.15 mg/L KIN. Crozier explants gave rise to shoots in MS medium supplemented with 0.5 mg/L BA. Direct regeneration of plantlets was obtained from rhizome explants supplemented with 0.25 mg/L BA and crozier explant cultured on MS medium supplemented with 0.5 mg/L IBA. Neither callogenesis nor organogenesis was observed from gametophyte explants. This study showed that A. capillus-veneris can be raised from spores. A protocol for efficient regeneration of A. capillus-veneris has been developed using rhizome explant in BA and crozier segment in IBA. 2019-06-25T10:12:36Z 2019-06-25T10:12:36Z 2014 Thesis Olatunji, T.Y. (2014). Regeneration of Adiantum Capillus-Veneris L.: an homosporous fern species. https://ir.oauife.edu.ng/handle/123456789/4287 en application/pdf Obafemi Awolowo University
spellingShingle Regeneration of Adiantum Capillus-Veneris
Homosporous Fern Species
Spore and Tissues
Vegetative Propagation
Parker's and Thompson's Nutrient
Fern
Medicinal fern species
Pteridophyte
Pteridophytes
Adiantum Capillus-Veneris
Olatunji, Tosin Yetunde
Regeneration of Adiantum Capillus-Veneris L.: an homosporous fern species.
title Regeneration of Adiantum Capillus-Veneris L.: an homosporous fern species.
title_full Regeneration of Adiantum Capillus-Veneris L.: an homosporous fern species.
title_fullStr Regeneration of Adiantum Capillus-Veneris L.: an homosporous fern species.
title_full_unstemmed Regeneration of Adiantum Capillus-Veneris L.: an homosporous fern species.
title_short Regeneration of Adiantum Capillus-Veneris L.: an homosporous fern species.
title_sort regeneration of adiantum capillus veneris l an homosporous fern species
topic Regeneration of Adiantum Capillus-Veneris
Homosporous Fern Species
Spore and Tissues
Vegetative Propagation
Parker's and Thompson's Nutrient
Fern
Medicinal fern species
Pteridophyte
Pteridophytes
Adiantum Capillus-Veneris
url https://ir.oauife.edu.ng/handle/123456789/4287
work_keys_str_mv AT olatunjitosinyetunde regenerationofadiantumcapillusvenerislanhomosporousfernspecies