Effect of dietary restriction on Longetivity,Survival and Fecundity of the blowfly chrysomya chloropyga(Wied.)(Diptera:Calliphoridea).
xvii,109p
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| Formatua: | Thesis |
| Hizkuntza: | ingelesa |
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zoology,Obafemi Awolowo University
2023
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| Sarrera elektronikoa: | https://ir.oauife.edu.ng/123456789/5402 |
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| _version_ | 1810764574789468160 |
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| author | Naman, Kasang |
| author_facet | Naman, Kasang |
| author_sort | Naman, Kasang |
| collection | DSpace |
| description | xvii,109p |
| format | Thesis |
| id | oai:ir.oauife.edu.ng:123456789-5402 |
| institution | My University |
| language | English |
| publishDate | 2023 |
| publisher | zoology,Obafemi Awolowo University |
| record_format | dspace |
| spelling | oai:ir.oauife.edu.ng:123456789-54022023-05-13T18:12:13Z Effect of dietary restriction on Longetivity,Survival and Fecundity of the blowfly chrysomya chloropyga(Wied.)(Diptera:Calliphoridea). Naman, Kasang Dietary restriction Longetivity Fecundity Blowfly Survival xvii,109p The effect of dietary restriction (DR) on longevity, survival and fecundity of the blowfly Chrysomya chloropyga was examined. The study also examined the protein content and the protein profiles of the blowflies on restricted diet and its effects on ovarian development. This was with a view to determining the effect of DR on the age of the fly at first egg laying. Laboratory reared adult blowflies were distributed into five cages (40 × 30 × 30 cm3) containing 20 males and 20 females each and thereafter exposed to four dietary restrictions. The continuous feeding cage served as the (Control), while other blowflies were subjected to 24, 48, 72 and 96 hr dietary restrictions in the insectary. The flies were fed on a mixture of ground rice and fish paste for an hour after every restriction periods. Sugar and water were provided ad libitum. Survival was determined by recording mortality daily and longevity by the maximum number of days lived. Insect weights were taken every 72 hr. Eggs laid by the control females and females exposed to 24, 48, 72 and 96 hr dietary restrictions were removed and counted to determine the mean fecundity. Three control females and those exposed to various periods of dietary restriction were sacrificed and dissected on day 18 to determine the ovarian development. Whole body homogenate of males and females from each of the dietary restricted groups and control were prepared by homogenizing the flies with 1ml distilled water and the total protein content of the supernatant samples were determined. The protein profile of males and females were also determined on the whole body homogenate by sodium deodecyl-sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Dietary restriction shortened the life span of the adult blowfly C. chloropyga compared with flies on continuous diet. Maximum longevity of flies fed ad libitum (control) was 59 and 67 days for males and females respectively. Maximum longevity of males and females under 24 hr restriction were 44 and 45 days, and 38 and 33 for those under 96 hr restriction. Weights of males and females decreased with increase in exposure to DR and with age. There was no egg laying in the dietary restricted females and fecundity was 106.8 ± 9.12 eggs in the control females with the first egg laying occurring at day 11. The lack of egg laying was evident by the undeveloped ovaries of females exposed to 24, 48, 72 and 96 hr dietary restriction. Protein concentrations of whole body homogenate of the dietary restricted males and females were lower at ages 5, 20 and 30 days and with increase in periods of exposure when compared with flies on continuous feeding. SDS- PAGE of whole body homogenate of males and females revealed the presence of wide range of similar protein fractions ranging between 3 and 183 kD in the exposed and unexposed males and females. Statistical analysis showed that dietary restriction did not significantly (p ˃ 0.05) affects the protein components of the adults. The present study concluded that dietary restriction affected some physiological activities in males and females C. chloropyga, which shortened the life span. 2023-05-13T16:54:23Z 2023-05-13T16:54:23Z 2014 Thesis Naman,K.(2014). Effect of dietary restriction on Longetivity,Survival and Fecundity of the blowfly chrysomya chloropyga(Wied.)(Diptera:Calliphoridea). Obafemi Awolowo University https://ir.oauife.edu.ng/123456789/5402 en application/pdf zoology,Obafemi Awolowo University |
| spellingShingle | Dietary restriction Longetivity Fecundity Blowfly Survival Naman, Kasang Effect of dietary restriction on Longetivity,Survival and Fecundity of the blowfly chrysomya chloropyga(Wied.)(Diptera:Calliphoridea). |
| title | Effect of dietary restriction on Longetivity,Survival and Fecundity of the blowfly chrysomya chloropyga(Wied.)(Diptera:Calliphoridea). |
| title_full | Effect of dietary restriction on Longetivity,Survival and Fecundity of the blowfly chrysomya chloropyga(Wied.)(Diptera:Calliphoridea). |
| title_fullStr | Effect of dietary restriction on Longetivity,Survival and Fecundity of the blowfly chrysomya chloropyga(Wied.)(Diptera:Calliphoridea). |
| title_full_unstemmed | Effect of dietary restriction on Longetivity,Survival and Fecundity of the blowfly chrysomya chloropyga(Wied.)(Diptera:Calliphoridea). |
| title_short | Effect of dietary restriction on Longetivity,Survival and Fecundity of the blowfly chrysomya chloropyga(Wied.)(Diptera:Calliphoridea). |
| title_sort | effect of dietary restriction on longetivity survival and fecundity of the blowfly chrysomya chloropyga wied diptera calliphoridea |
| topic | Dietary restriction Longetivity Fecundity Blowfly Survival |
| url | https://ir.oauife.edu.ng/123456789/5402 |
| work_keys_str_mv | AT namankasang effectofdietaryrestrictiononlongetivitysurvivalandfecundityoftheblowflychrysomyachloropygawieddipteracalliphoridea |