Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria
international journal of Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump;138 page
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International Journal of Biological Research
2023
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| Acceso en línea: | https://ir.oauife.edu.ng/123456789/5476 |
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| author | Omoboye, Olumide Kolawole, Bakare Mufutau Olusanjo, Adewale Isaac Oyedeji, Olaoluwa |
| author_facet | Omoboye, Olumide Kolawole, Bakare Mufutau Olusanjo, Adewale Isaac Oyedeji, Olaoluwa |
| author_sort | Omoboye, Olumide |
| collection | DSpace |
| description | international journal of Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump;138 page |
| format | Journal |
| id | oai:ir.oauife.edu.ng:123456789-5476 |
| institution | My University |
| language | English |
| publishDate | 2023 |
| publisher | International Journal of Biological Research |
| record_format | dspace |
| spelling | oai:ir.oauife.edu.ng:123456789-54762023-05-13T17:46:46Z Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria Omoboye, Olumide Kolawole, Bakare Mufutau Olusanjo, Adewale Isaac Oyedeji, Olaoluwa Optimum Soluble Starch Thermophilic Alpha Amylase Bacillus Species international journal of Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump;138 page Molecular identification and amylolytic potential of a thermophilic bacterium species isolated from refuse dump was investigated. Bacte-rial isolates were identified by morphological and biochemical characterization while amylolytic bacterium of interest was identified by molecular analysis using 16S rRNA gene sequencing. The bacterium with the highest α-amylase activity was selected for enzyme pro-duction. The optimal conditions for α-amylase secretion were determined by varying the pH, temperature, percentage soluble starch, nitrogen sources and carbon sources. The isolated and identified bacteria were Bacillus alvei (40%) Bacillus licheniformis (40%) and Bacillus brevis (20%) while Bacillus licheniformis RD24 was identified by 16S rRNA gene sequencing. The peak of amylase production was at 20 h of incubation (925 µg/ml/min). The optimum pH and temperature for the enzyme production were 7 and 45oC respectively. Enzyme production medium with 1% starch gave highest enzyme activity of 102 ± 5.3 µg/ml/min. Peptone gave an enzyme activity of 165 ± 8.97 µg/ml/min and yeast extract gave 52.26 ± 2.86 µg/ml/min. Of the raw starches, cassava flour gave the highest specific activity of 72 ± 0.07 Units/mg proteins, while sorghum starch gave the lowest specific activity of 5 ± 1.52 Units/mg proteins. The study conclud-ed that starch-rich household waste can be employed for amylase production using Bacillus licheniformis RD24. 2023-05-13T17:46:46Z 2023-05-13T17:46:46Z 2014-10 Journal DOI: 10.14419 https://ir.oauife.edu.ng/123456789/5476 en text/plain International Journal of Biological Research |
| spellingShingle | Optimum Soluble Starch Thermophilic Alpha Amylase Bacillus Species Omoboye, Olumide Kolawole, Bakare Mufutau Olusanjo, Adewale Isaac Oyedeji, Olaoluwa Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria |
| title | Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria |
| title_full | Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria |
| title_fullStr | Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria |
| title_full_unstemmed | Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria |
| title_short | Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria |
| title_sort | molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in ile ife nigeria |
| topic | Optimum Soluble Starch Thermophilic Alpha Amylase Bacillus Species |
| url | https://ir.oauife.edu.ng/123456789/5476 |
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