Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria
Molecular identification and amylolytic potential of a thermophilic bacterium species isolated from refuse dump was investigated. Bacte-rial isolates were identified by morphological and biochemical characterization while amylolytic bacterium of interest was identified by molecular analysis using 16...
שמור ב:
| Main Authors: | , , , |
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| פורמט: | כתב-עת |
| שפה: | אנגלית |
| יצא לאור: |
journal of molecular
2023
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| נושאים: | |
| גישה מקוונת: | https://ir.oauife.edu.ng/123456789/5520 |
| תגים: |
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| סיכום: | Molecular identification and amylolytic potential of a thermophilic bacterium species isolated from refuse dump was investigated. Bacte-rial isolates were identified by morphological and biochemical characterization while amylolytic bacterium of interest was identified by molecular analysis using 16S rRNA gene sequencing. The bacterium with the highest α-amylase activity was selected for enzyme pro-duction. The optimal conditions for α-amylase secretion were determined by varying the pH, temperature, percentage soluble starch, nitrogen sources and carbon sources. The isolated and identified bacteria were Bacillus alvei (40%) Bacillus licheniformis (40%) and Bacillus brevis (20%) while Bacillus licheniformis RD24 was identified by 16S rRNA gene sequencing. The peak of amylase production was at 20 h of incubation (925 μg/ml/min). The optimum pH and temperature for the enzyme production were 7 and 45oC respectively. Enzyme production medium with 1% starch gave highest enzyme activity of 102 ± 5.3 μg/ml/min. Peptone gave an enzyme activity of 165 ± 8.97 μg/ml/min and yeast extract gave 52.26 ± 2.86 μg/ml/min. Of the raw starches, cassava flour gave the highest specific activity of 72 ± 0.07 Units/mg proteins, while sorghum starch gave the lowest specific activity of 5 ± 1.52 Units/mg proteins. The study conclud-ed that starch-rich household waste can be employed for amylase production using Bacillus licheniformis RD24 |
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