Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria
Molecular identification and amylolytic potential of a thermophilic bacterium species isolated from refuse dump was investigated. Bacte-rial isolates were identified by morphological and biochemical characterization while amylolytic bacterium of interest was identified by molecular analysis using 16...
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journal of molecular
2023
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| Ábhair: | |
| Rochtain ar líne: | https://ir.oauife.edu.ng/123456789/5520 |
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| _version_ | 1810764576151568384 |
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| author | Omoboye, Olumide Kolawole, Bakare Mufutau Olusanjo, Adewale Isaac Olaoluwa, Oyedeji |
| author_facet | Omoboye, Olumide Kolawole, Bakare Mufutau Olusanjo, Adewale Isaac Olaoluwa, Oyedeji |
| author_sort | Omoboye, Olumide |
| collection | DSpace |
| description | Molecular identification and amylolytic potential of a thermophilic bacterium species isolated from refuse dump was investigated. Bacte-rial isolates were identified by morphological and biochemical characterization while amylolytic bacterium of interest was identified by molecular analysis using 16S rRNA gene sequencing. The bacterium with the highest α-amylase activity was selected for enzyme pro-duction. The optimal conditions for α-amylase secretion were determined by varying the pH, temperature, percentage soluble starch, nitrogen sources and carbon sources. The isolated and identified bacteria were Bacillus alvei (40%) Bacillus licheniformis (40%) and Bacillus brevis (20%) while Bacillus licheniformis RD24 was identified by 16S rRNA gene sequencing. The peak of amylase production was at 20 h of incubation (925 μg/ml/min). The optimum pH and temperature for the enzyme production were 7 and 45oC respectively. Enzyme production medium with 1% starch gave highest enzyme activity of 102 ± 5.3 μg/ml/min. Peptone gave an enzyme activity of 165 ± 8.97 μg/ml/min and yeast extract gave 52.26 ± 2.86 μg/ml/min. Of the raw starches, cassava flour gave the highest specific activity of 72 ± 0.07 Units/mg proteins, while sorghum starch gave the lowest specific activity of 5 ± 1.52 Units/mg proteins. The study conclud-ed that starch-rich household waste can be employed for amylase production using Bacillus licheniformis RD24 |
| format | Journal |
| id | oai:ir.oauife.edu.ng:123456789-5520 |
| institution | My University |
| language | English |
| publishDate | 2023 |
| publisher | journal of molecular |
| record_format | dspace |
| spelling | oai:ir.oauife.edu.ng:123456789-55202023-05-13T17:52:18Z Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria Omoboye, Olumide Kolawole, Bakare Mufutau Olusanjo, Adewale Isaac Olaoluwa, Oyedeji amylolytic thermophilic bacteria Molecular nitrogen Molecular identification and amylolytic potential of a thermophilic bacterium species isolated from refuse dump was investigated. Bacte-rial isolates were identified by morphological and biochemical characterization while amylolytic bacterium of interest was identified by molecular analysis using 16S rRNA gene sequencing. The bacterium with the highest α-amylase activity was selected for enzyme pro-duction. The optimal conditions for α-amylase secretion were determined by varying the pH, temperature, percentage soluble starch, nitrogen sources and carbon sources. The isolated and identified bacteria were Bacillus alvei (40%) Bacillus licheniformis (40%) and Bacillus brevis (20%) while Bacillus licheniformis RD24 was identified by 16S rRNA gene sequencing. The peak of amylase production was at 20 h of incubation (925 μg/ml/min). The optimum pH and temperature for the enzyme production were 7 and 45oC respectively. Enzyme production medium with 1% starch gave highest enzyme activity of 102 ± 5.3 μg/ml/min. Peptone gave an enzyme activity of 165 ± 8.97 μg/ml/min and yeast extract gave 52.26 ± 2.86 μg/ml/min. Of the raw starches, cassava flour gave the highest specific activity of 72 ± 0.07 Units/mg proteins, while sorghum starch gave the lowest specific activity of 5 ± 1.52 Units/mg proteins. The study conclud-ed that starch-rich household waste can be employed for amylase production using Bacillus licheniformis RD24 2023-05-13T17:52:18Z 2023-05-13T17:52:18Z 2014-10 Journal DOI: 10.14419/ijbr.v2i2.2537 https://ir.oauife.edu.ng/123456789/5520 en text/plain text/plain text/plain journal of molecular |
| spellingShingle | amylolytic thermophilic bacteria Molecular nitrogen Omoboye, Olumide Kolawole, Bakare Mufutau Olusanjo, Adewale Isaac Olaoluwa, Oyedeji Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria |
| title | Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria |
| title_full | Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria |
| title_fullStr | Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria |
| title_full_unstemmed | Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria |
| title_short | Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria |
| title_sort | molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in ile ife nigeria |
| topic | amylolytic thermophilic bacteria Molecular nitrogen |
| url | https://ir.oauife.edu.ng/123456789/5520 |
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