An assessment on DNA microarray and sequence-based methods for the characterization of methicillin-susceptible Staphylococcus aureus from Nigeria
Frontiers in Microbiology,Vol. 6,Article 1160,p.10
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Frontiers in Microbiology
2023
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| author | Shittu, Adebayo O Oyedara, Omotayo Onko, Kenneth Raji, Adeola Peters, Georg Müller, Lutz von Schaumburg, Frieder Herrmann, Mathias Ruffing, Ulla |
| author_facet | Shittu, Adebayo O Oyedara, Omotayo Onko, Kenneth Raji, Adeola Peters, Georg Müller, Lutz von Schaumburg, Frieder Herrmann, Mathias Ruffing, Ulla |
| author_sort | Shittu, Adebayo O |
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| description | Frontiers in Microbiology,Vol. 6,Article 1160,p.10 |
| format | Journal |
| id | oai:ir.oauife.edu.ng:123456789-5530 |
| institution | My University |
| language | English |
| publishDate | 2023 |
| publisher | Frontiers in Microbiology |
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| spelling | oai:ir.oauife.edu.ng:123456789-55302023-05-13T18:07:37Z An assessment on DNA microarray and sequence-based methods for the characterization of methicillin-susceptible Staphylococcus aureus from Nigeria Shittu, Adebayo O Oyedara, Omotayo Onko, Kenneth Raji, Adeola Peters, Georg Müller, Lutz von Schaumburg, Frieder Herrmann, Mathias Ruffing, Ulla Nigeria genotyping MLST microarray Staphylococcus aureus Frontiers in Microbiology,Vol. 6,Article 1160,p.10 Staphylococcus aureus is an important human pathogen causing nosocomial and community-acquired infections worldwide. In the characterization of this opportunistic pathogen, DNA microarray hybridization technique is used as an alternative to sequence based genotyping to obtain a comprehensive assessment on the virulence, resistance determinants, and population structure. The objective of this study was to characterize a defined collection of S. aureus isolates from Nigeria using the microarray technique, and to assess the extent that it correlates with sequence-based genotyping methods. The clonal diversity and genomic content of 52 methicillin-susceptible Staphylococcus aureus (MSSA)wereinvestigatedby spa typing,MLSTandDNAmicroarrayhybridization. More than half (55.8%) of these isolates were associated with clonal complexes (CCs) typically associated with methicillin-resistant S. aureus (MRSA) clones i.e., CC1, CC5, CC8,CC30,andCC45.Certaingeneslinkedwithvirulence(hlgAandclfA)andadherence (ebpS, fnbA, sspA, sspB, and sspP) were detected in all isolates. A number of genes or gene clusters were associated with distinct clonal types. The enterotoxin gene cluster (egc) was linked with CC5, CC25, CC30, CC45, and CC121, enterotoxin H gene (seh) with CC1, exfoliative toxin D gene (etd) with CC25 and CC80, and the epidermal cell differentiation inhibitor B gene (edinB) with CC25, CC80, and CC152. The excellent agreement between data from DNA microarray and MLST in the delineation of Nigerian MSSA isolates indicates that the microarray technique is a useful tool to provide information on antibiotic resistance, clonal diversity and virulence factors associated with infection and disease. 2023-05-13T17:53:43Z 2023-05-13T17:53:43Z 2015-10-20 Journal 10.3389/fmicb.2015.01160 https://ir.oauife.edu.ng/123456789/5530 en application/pdf Frontiers in Microbiology |
| spellingShingle | Nigeria genotyping MLST microarray Staphylococcus aureus Shittu, Adebayo O Oyedara, Omotayo Onko, Kenneth Raji, Adeola Peters, Georg Müller, Lutz von Schaumburg, Frieder Herrmann, Mathias Ruffing, Ulla An assessment on DNA microarray and sequence-based methods for the characterization of methicillin-susceptible Staphylococcus aureus from Nigeria |
| title | An assessment on DNA microarray and sequence-based methods for the characterization of methicillin-susceptible Staphylococcus aureus from Nigeria |
| title_full | An assessment on DNA microarray and sequence-based methods for the characterization of methicillin-susceptible Staphylococcus aureus from Nigeria |
| title_fullStr | An assessment on DNA microarray and sequence-based methods for the characterization of methicillin-susceptible Staphylococcus aureus from Nigeria |
| title_full_unstemmed | An assessment on DNA microarray and sequence-based methods for the characterization of methicillin-susceptible Staphylococcus aureus from Nigeria |
| title_short | An assessment on DNA microarray and sequence-based methods for the characterization of methicillin-susceptible Staphylococcus aureus from Nigeria |
| title_sort | assessment on dna microarray and sequence based methods for the characterization of methicillin susceptible staphylococcus aureus from nigeria |
| topic | Nigeria genotyping MLST microarray Staphylococcus aureus |
| url | https://ir.oauife.edu.ng/123456789/5530 |
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