Archachatina (Calachatina) Marginata Haemolymph Proteins, Physicochemical Characterization of Protein B.

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Main Author: Binutu, Olaoluwa Olujare
Format: Thesis
Language:English
Published: Obafemi Awolowo University, 2023
Subjects:
Online Access:https://ir.oauife.edu.ng/123456789/5631
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author Binutu, Olaoluwa Olujare
author_facet Binutu, Olaoluwa Olujare
author_sort Binutu, Olaoluwa Olujare
collection DSpace
description 75P
format Thesis
id oai:ir.oauife.edu.ng:123456789-5631
institution My University
language English
publishDate 2023
publisher Obafemi Awolowo University,
record_format dspace
spelling oai:ir.oauife.edu.ng:123456789-56312023-05-13T18:11:08Z Archachatina (Calachatina) Marginata Haemolymph Proteins, Physicochemical Characterization of Protein B. Binutu, Olaoluwa Olujare Haemolymph Ultracentrifugation/ Physicochemical Carboxymethylated/ Peptide 75P The haemolymph of Archachatina (Calachatina) marginata has been found to contain other proteins apart from the predominant protein, haemocyanin. One of the other protein components, Protein B, was isolated and purified using preparatory ultracentrifugation and gel filtration techniques. Physicochemical characterization, employing different techniques has showe that Protein B is different from the haemocyanin (and/or its subunit(s)) from this mollusc. Protein B has a molecular weight of 360KD consisting of two chains which are of identical molecular size. Amino acid composition for Protein B shows that: (i)there exist more acidic residues combined than those of the basic residues combined. (ii) there is a very large occurence of Proline residues (iii) there is also a large amount of cysteine residues. There is one gram atom of copper per dimer. Protein B is a glycoprotein. The carbohydrate portion is made up of units of acetylglucosamine and galactosamine. Peptic peptide fractionation of reduced carboxymethylated Protein B has shown that most of the carbohydrate can be found on a peptide having a mass of 15KD. 2023-05-13T18:03:13Z 2023-05-13T18:03:13Z 1985 Thesis Binutu, O.O Archachatina (Calachatina) Marginata Haemolymph proteins, physicochemical characterization of protein B. https://ir.oauife.edu.ng/123456789/5631 en application/pdf Obafemi Awolowo University,
spellingShingle Haemolymph
Ultracentrifugation/ Physicochemical
Carboxymethylated/
Peptide
Binutu, Olaoluwa Olujare
Archachatina (Calachatina) Marginata Haemolymph Proteins, Physicochemical Characterization of Protein B.
title Archachatina (Calachatina) Marginata Haemolymph Proteins, Physicochemical Characterization of Protein B.
title_full Archachatina (Calachatina) Marginata Haemolymph Proteins, Physicochemical Characterization of Protein B.
title_fullStr Archachatina (Calachatina) Marginata Haemolymph Proteins, Physicochemical Characterization of Protein B.
title_full_unstemmed Archachatina (Calachatina) Marginata Haemolymph Proteins, Physicochemical Characterization of Protein B.
title_short Archachatina (Calachatina) Marginata Haemolymph Proteins, Physicochemical Characterization of Protein B.
title_sort archachatina calachatina marginata haemolymph proteins physicochemical characterization of protein b
topic Haemolymph
Ultracentrifugation/ Physicochemical
Carboxymethylated/
Peptide
url https://ir.oauife.edu.ng/123456789/5631
work_keys_str_mv AT binutuolaoluwaolujare archachatinacalachatinamarginatahaemolymphproteinsphysicochemicalcharacterizationofproteinb