THE USE OF SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS IN FISH BLOOD SERUM ANALYSIS
This study considered the use of Native-Polyacrylamide Gel Electrophoresis (N- PAGE) and Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) in analyzing fish blood serum protein as a tool in aquaculture management. Blood samples were obtained from African catfish (Clarias gariepin...
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| Формат: | Online |
| Мова: | Англійська |
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The Faculty of Agriculture Obafemi Awolowo University, Ile-Ife, Nigeria.
2020
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| Онлайн доступ: | https://ija.oauife.edu.ng/index.php/ija/article/view/383 |
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| _version_ | 1810820075018518528 |
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| author | BETIKU, O. C. |
| author_facet | BETIKU, O. C. |
| author_sort | BETIKU, O. C. |
| collection | OJS |
| description | This study considered the use of Native-Polyacrylamide Gel Electrophoresis (N- PAGE) and Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) in analyzing fish blood serum protein as a tool in aquaculture management. Blood samples were obtained from African catfish (Clarias gariepinus) collected from Niger State, Nigeria. Serum protein obtained from the blood samples were analyzed using 5.5% of N-PAGE and 5.5, 7, 8, 10 and 12% SDS-PAGE. The resulting gels from 12% SDS-PAGE analysis showed better resolution of the protein bands compared to 10% SDS-PAGE, while 5.5%, 7% and 8% SDS PAGE did not show the presence of any protein band. In addition, 12% SDS-PAGE showed better resolution of the fish serum samples with more protein bands than N-PAGE. This observation could be useful in analyzing fish serum protein for generating dendrogram grouping in determining the relationship between fish from the same location or between hybrid and parent stocks. |
| format | Online |
| id | oai:ojs2.ija.oauife.edu.ng:article-383 |
| institution | My University |
| language | eng |
| publishDate | 2020 |
| publisher | The Faculty of Agriculture Obafemi Awolowo University, Ile-Ife, Nigeria. |
| record_format | ojs |
| spelling | oai:ojs2.ija.oauife.edu.ng:article-3832020-09-07T14:41:06Z THE USE OF SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS IN FISH BLOOD SERUM ANALYSIS BETIKU, O. C. Blood serum, N-PAGE, SDS PAGE, Clarias gariepinus This study considered the use of Native-Polyacrylamide Gel Electrophoresis (N- PAGE) and Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) in analyzing fish blood serum protein as a tool in aquaculture management. Blood samples were obtained from African catfish (Clarias gariepinus) collected from Niger State, Nigeria. Serum protein obtained from the blood samples were analyzed using 5.5% of N-PAGE and 5.5, 7, 8, 10 and 12% SDS-PAGE. The resulting gels from 12% SDS-PAGE analysis showed better resolution of the protein bands compared to 10% SDS-PAGE, while 5.5%, 7% and 8% SDS PAGE did not show the presence of any protein band. In addition, 12% SDS-PAGE showed better resolution of the fish serum samples with more protein bands than N-PAGE. This observation could be useful in analyzing fish serum protein for generating dendrogram grouping in determining the relationship between fish from the same location or between hybrid and parent stocks. The Faculty of Agriculture Obafemi Awolowo University, Ile-Ife, Nigeria. 2020-09-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ija.oauife.edu.ng/index.php/ija/article/view/383 Ife Journal of Agriculture; Vol. 24 No. 1 (2009): Ife Journal of Agriculture; 123-131 0331-6351 eng https://ija.oauife.edu.ng/index.php/ija/article/view/383/274 Copyright (c) 2020 Ife Journal of Agriculture |
| spellingShingle | Blood serum, N-PAGE, SDS PAGE, Clarias gariepinus BETIKU, O. C. THE USE OF SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS IN FISH BLOOD SERUM ANALYSIS |
| title | THE USE OF SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS IN FISH BLOOD SERUM ANALYSIS |
| title_full | THE USE OF SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS IN FISH BLOOD SERUM ANALYSIS |
| title_fullStr | THE USE OF SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS IN FISH BLOOD SERUM ANALYSIS |
| title_full_unstemmed | THE USE OF SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS IN FISH BLOOD SERUM ANALYSIS |
| title_short | THE USE OF SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS IN FISH BLOOD SERUM ANALYSIS |
| title_sort | use of sodium dodecyl sulfate polyacrylamide gel electrophoresis in fish blood serum analysis |
| topic | Blood serum, N-PAGE, SDS PAGE, Clarias gariepinus |
| topic_facet | Blood serum, N-PAGE, SDS PAGE, Clarias gariepinus |
| url | https://ija.oauife.edu.ng/index.php/ija/article/view/383 |
| work_keys_str_mv | AT betikuoc theuseofsodiumdodecylsulfatepolyacrylamidegelelectrophoresisinfishbloodserumanalysis AT betikuoc useofsodiumdodecylsulfatepolyacrylamidegelelectrophoresisinfishbloodserumanalysis |